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Chronic constipation, defined as persistent symptoms of difficult defecation, reduced bowel movement frequency, or incomplete evacuation lasting at least 3 months, has a global prevalence of approximately 1415%, with considerable variation across regions and populations (Bharucha and Lacy, 2020)

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The next day, 2 ml of each of the cultures was added to 100 ml LB liquid medium containing 100 mg/L of ampicillin and allowed to grow at 37 until the optical density at 600 nm (OD 600 ) reaches between 0.4 and 0.6